Stylissamide B was successfully synthesized for the first time using Fmoc-SPPS monitored using ReD-A method followed by cyclization in solution phase. The linear peptide, Pro-Pro-Ile-Tyr-Pro-Phe-Pro, was cyclized to form stylissamide B. It is noted that position of the Pro-Pro-Pro motif affects cyclization efficiency.
ABSTRACT
Stylissamide B was successfully synthesized for the first time using Fmoc solid-phase peptide synthesis (Fmoc-SPPS) followed by cyclization in solution phase. The Fmoc-SPPS process was monitored using the reversible detection method for amino groups (ReD-A method). The linear peptide, Pro-Pro-Ile-Tyr-Pro-Phe-Pro, was cyclized to form stylissamide B. The spectral data obtained were in agreement with previously reported literature, thereby confirming the reliability of the synthetic method.
